cervix human cancer cell line hela  (ATCC)

Journal: Bioengineered

Article Title: Overexpression of Eukaryotic translation initiation factor 3D induces stem cell-like properties and metastasis in cervix cancer by activating FAK through inhibiting degradation of GRP78

doi: 10.1080/21655979.2021.2024336

Figure Lengend Snippet: EIF3D had high expression in human cervix cancer tissues and cells. (a). qPCR assays showed the mRNA levels of EIF3D in cervix cancer tissues and normal tissues (b). Immunoblot assays showed the protein levels of EIF3D in three representative cervix cancer tissues and corresponding normal tissues (c). qPCR assays showed the mRNA levels of EIF3D in cervix cancer cell lines including HeLa, CaSKi, and SiHa, and normal cervix cell line H8. (d). Immunoblot assays showed the protein levels of EIF3D in cervix cancer cell lines and normal cervix cell line. (e). IHC assays showed EIF3D expression in tumor tissues and normal tissues from cervix cancer patients. Data are shown as mean ± SEM, ** p < 0.01.

Article Snippet: 3 types of human cervix cancer cell lines HeLa, CaSKi, and SiHa, and 1 type of normal cervix cell line H8, were purchased from ATCC.

Techniques: Expressing, Western Blot

Journal: Frontiers in Oncology

Article Title: Cancer Cells Can Exhibit a Sparing FLASH Effect at Low Doses Under Normoxic In Vitro- Conditions

doi: 10.3389/fonc.2021.686142

Figure Lengend Snippet: Surviving fraction assessed by clonogenic assay comparing FLASH with conventional dose rates (CONV) for human in vitro- cell lines; (A) Breast cancer cell line MDA-MB-231, (B) Breast cancer cell line MCF7, (C) Cervix cancer cell line HeLa early passage , (D) HeLa subclone (E) Head&neck cancer cell line LU-HNSCC4, (F) Colon cancer cell line WiDr, and (G) Normal lung fibroblasts MRC-5. Blue circles denote FLASH, red squares denote CONV, and grey circles denote the non-irradiated controls. The empty symbols represent the individual flasks and the filled symbols represent the average surviving fraction at the dose indicated. The dashed lines illustrate the fitted survival curve according to the linear quadratic model. Diamond symbols denote samples below the detection limit (no surviving colonies). Statistical analyses using Wilcoxon Rank-Sum test; ns, not significant, *p < 0.05, **p < 0.01. Data from three independent experiments.

Article Snippet: The human breast cancer cell lines MCF7 and MDA-MB-231, the human fibroblast cell line MRC-5, and the human cervix cancer cell line HeLa (in the study two different HeLa cells were used; early passage cells and a high passage subclone) were acquired from American Type Culture Collection (ATCC).

Techniques: Clonogenic Assay, In Vitro, Irradiation

Journal: Frontiers in Oncology

Article Title: Cancer Cells Can Exhibit a Sparing FLASH Effect at Low Doses Under Normoxic In Vitro- Conditions

doi: 10.3389/fonc.2021.686142

Figure Lengend Snippet: Dose modifying factors (DMF) at a surviving fraction (SF) of 0.1 and 0.01 for the various cell lines.

Article Snippet: The human breast cancer cell lines MCF7 and MDA-MB-231, the human fibroblast cell line MRC-5, and the human cervix cancer cell line HeLa (in the study two different HeLa cells were used; early passage cells and a high passage subclone) were acquired from American Type Culture Collection (ATCC).

Techniques:

Journal: Frontiers in Oncology

Article Title: Cancer Cells Can Exhibit a Sparing FLASH Effect at Low Doses Under Normoxic In Vitro- Conditions

doi: 10.3389/fonc.2021.686142

Figure Lengend Snippet: Evaluation of radiation-induced DNA-double strand break foci using 53BP1. (A) Representative microscopy image showing (left to right) 53BP1, DAPI, merged image, and the resulting analyzed image after processing in ImageJ. (B–D) Number of 53BP1 foci at 2 h and 24 h after 3 Gy irradiation with FLASH (blue) or conventional dose rate (CONV, red) compared with controls (grey) for LU-HNSCC4 ( B ; 1,532 scored cells), MDA-MB-231 ( C ; 2,583 scored cells), and HeLa subclone ( D ; 2,973 scored cells). The box and whisker plots illustrate median (thick line), interquartile range (box) and the lowest/highest observation within ±1.5* interquartile range (IQR) from the box (whiskers). The individually scored cells are indicated with transparent circles. Data from two independent experiments.

Article Snippet: The human breast cancer cell lines MCF7 and MDA-MB-231, the human fibroblast cell line MRC-5, and the human cervix cancer cell line HeLa (in the study two different HeLa cells were used; early passage cells and a high passage subclone) were acquired from American Type Culture Collection (ATCC).

Techniques: Microscopy, Irradiation, Whisker Assay

Journal: Frontiers in Oncology

Article Title: Cancer Cells Can Exhibit a Sparing FLASH Effect at Low Doses Under Normoxic In Vitro- Conditions

doi: 10.3389/fonc.2021.686142

Figure Lengend Snippet: Cell cycle distributions determined by flow cytometry after irradiation with FLASH or conventional dose rate (CONV). In (A–C) , cell cycle distribution 24 h after irradiation with 6 Gy for LU-HNSCC4 (A) , MDA-MB-231 (B) and HeLa subclone (C) . In (D) an earlier time-point (6 h) after irradiation with 3 Gy and 6 Gy using the HeLa subclone . Bars illustrate G1 (light grey), S-phase (grey), and G2/M (black). The figures in the bars denote the percentage of cells (with standard deviations). Data from two independent experiments.

Article Snippet: The human breast cancer cell lines MCF7 and MDA-MB-231, the human fibroblast cell line MRC-5, and the human cervix cancer cell line HeLa (in the study two different HeLa cells were used; early passage cells and a high passage subclone) were acquired from American Type Culture Collection (ATCC).

Techniques: Flow Cytometry, Irradiation